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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med (2009) 2, 254-265
Original Article
Oxidative markers, nitric oxide and homocysteine alteration in
hypercholesterolimic rats: role of atorvastatine and cinnamon
Kamal A. Amin, Thanaa M. Abd El-Twab
Biochemistry Department, Faculty of Veterinary Medicine, Beni- Suef, University; Physiology, Zoology Department, Faculty of Science,
Beni-Suief University, Beni Suef, Egypt.
Received September 6, 2009; accepted September 28, 2009; available online October 5, 2009
Abstract: To investigate the effects of atorvastatin and cinnamon on serum lipid profile, oxidative stress, antioxidant capacity, hepatic
enzymes activities, nitric oxide (NO) as well as homocysteine (Hcy) in hypercholesterolemic rats, 48 male albino rats, weighing
130-190 gm were divided into 2 groups, normal group fed on basal rat chow diet (n=12) and high cholesterol group (HCD) were fed on
1% cholesterol-enriched diet for 15 day (n=36). Hypercholesterolemic rats were divided into 3 subgroups (n=12 for each) fed the same
diet and treated with atorvastatine (HCD+Atorvastatin) or cinnamon extract (HCD+cinnamon) or none treated (HCD) for 3&6 weeks.
Serum triglycerides (TG), Total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), ALT, AST, NO, Hcy,
hepatic reduced glutathione (GSH), Malondialdehyde (MDA) and antioxidant enzymes, Superoxide dismutase (SOD) and catalase
activity were measured. Results showed that HCD increased significantly TG, TC, LDL-C, ALT, AST, Hcy and hepatic MDA, while
lowered significantly antioxidant enzyme activities and NO levels. Atorvastatin therapy significantly increased HDL-C, NO and antioxidant
activity while decreased LDL-C, MDA and Hcy concentrations. Serum TG, TC, LDL-C, ALT, AST and hepatic MDA levels were
significantly lowered meanwhile, serum HDL, NO values and hepatic antioxidant activities were significantly, higher in
cinnamon-treated than untreated group. These results indicate that lipid abnormalities, oxidative injury and hyperhomocystienemia
were induced by HCD and this study recommend that administration of atorvastatine or cinnamon provided protection against the
lipemic-oxidative disorder and act as hypocholesterolemic, hepatoprotective agent and improve cardiovascular function through
modulation of oxidative stress, NO and Hcy. (IJCEM909004).
Key Words: Hypercholesterolemia, homocystein, nitric oxide, oxidative stress atorvastatin, cinnamon
Full Text PDF
Address all correspondence to:
Kamal A. Amin, PhD
Department of Biochemistry
Faculty of Veterinary medicine
Beni Suef University
Beni Suef 62511
Egypt
Tel/Fax: (+20)-082-2327982
E-mail: kaamin10@yahoo.com
Original Article
Oxidative markers, nitric oxide and homocysteine alteration in
hypercholesterolimic rats: role of atorvastatine and cinnamon
Kamal A. Amin, Thanaa M. Abd El-Twab
Biochemistry Department, Faculty of Veterinary Medicine, Beni- Suef, University; Physiology, Zoology Department, Faculty of Science,
Beni-Suief University, Beni Suef, Egypt.
Received September 6, 2009; accepted September 28, 2009; available online October 5, 2009
Abstract: To investigate the effects of atorvastatin and cinnamon on serum lipid profile, oxidative stress, antioxidant capacity, hepatic
enzymes activities, nitric oxide (NO) as well as homocysteine (Hcy) in hypercholesterolemic rats, 48 male albino rats, weighing
130-190 gm were divided into 2 groups, normal group fed on basal rat chow diet (n=12) and high cholesterol group (HCD) were fed on
1% cholesterol-enriched diet for 15 day (n=36). Hypercholesterolemic rats were divided into 3 subgroups (n=12 for each) fed the same
diet and treated with atorvastatine (HCD+Atorvastatin) or cinnamon extract (HCD+cinnamon) or none treated (HCD) for 3&6 weeks.
Serum triglycerides (TG), Total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL), ALT, AST, NO, Hcy,
hepatic reduced glutathione (GSH), Malondialdehyde (MDA) and antioxidant enzymes, Superoxide dismutase (SOD) and catalase
activity were measured. Results showed that HCD increased significantly TG, TC, LDL-C, ALT, AST, Hcy and hepatic MDA, while
lowered significantly antioxidant enzyme activities and NO levels. Atorvastatin therapy significantly increased HDL-C, NO and antioxidant
activity while decreased LDL-C, MDA and Hcy concentrations. Serum TG, TC, LDL-C, ALT, AST and hepatic MDA levels were
significantly lowered meanwhile, serum HDL, NO values and hepatic antioxidant activities were significantly, higher in
cinnamon-treated than untreated group. These results indicate that lipid abnormalities, oxidative injury and hyperhomocystienemia
were induced by HCD and this study recommend that administration of atorvastatine or cinnamon provided protection against the
lipemic-oxidative disorder and act as hypocholesterolemic, hepatoprotective agent and improve cardiovascular function through
modulation of oxidative stress, NO and Hcy. (IJCEM909004).
Key Words: Hypercholesterolemia, homocystein, nitric oxide, oxidative stress atorvastatin, cinnamon
Full Text PDF
Address all correspondence to:
Kamal A. Amin, PhD
Department of Biochemistry
Faculty of Veterinary medicine
Beni Suef University
Beni Suef 62511
Egypt
Tel/Fax: (+20)-082-2327982
E-mail: kaamin10@yahoo.com
