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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2(3):280-288,2009.
Original Article
DNA repair in Etoposide-induced DNA damage in lymphocytes of breast cancer
patients and healthy women
Ana Claudia Teixeira, Raquel Alves dos Santos, Aline Poersch, Helio Humberto Angotti Carrara, Jurandyr Moreira de Andrade, Catarina
Satie Takahashi
Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14040-901 Ribeirão
Preto, SP, Brazil; Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av.
Bandeirantes, 3900, 14040-900 Ribeirão Preto, SP, Brazil; Department of Biology, Faculty of Philosophy, Sciences and Letters of
Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14040-901 Ribeirão Preto, SP, Brazil.
Received September 3, 2009; accepted October 13, 2009; available online October 20, 2009
Abstract: The present study evaluated the basal DNA damage and the cellular response to this damage induced by in vitro
administration of Etoposide in lymphocytes donated by twenty untreated breast cancer (BC) patients and twenty age-matched healthy
women. Micronucleus (MN) and alkaline Comet Assays were performed in cultured peripheral blood lymphocytes (PBL) according to a
standard protocol for in vitro treatment with various concentrations of Etoposide or a control. For the Comet Assay, three samples of
cells were collected: T0 (immediately preceding treatment of the cultures), T1 (immediately after completion of the treatment) and T2
(four hours after completion of the treatment). MN frequency in the BC group treated with 25 µM Etoposide (19.1 ± 7.35) was
significantly higher than the control (10.9 ± 9.87) group. In the alkaline Comet Assay, both the BC group and the healthy women showed
the ability to repair Etoposide-induced DNA damage within 4 hours of reincubation. (IJCEM909002).
Key words: Micronucleus Test, comet Assay, breast cancer, DNA repair, Etoposide
Full Text PDF
Address all correspondence to:
Department of Genetics
Faculty of Medicine of Ribeirão Preto
University of São Paulo
Av. Bandeirantes, 3900, 14040-901
Ribeirão Preto, SP, Brazil.
E-mail: anaclaudia.teixeira@yahoo.com.br
Original Article
DNA repair in Etoposide-induced DNA damage in lymphocytes of breast cancer
patients and healthy women
Ana Claudia Teixeira, Raquel Alves dos Santos, Aline Poersch, Helio Humberto Angotti Carrara, Jurandyr Moreira de Andrade, Catarina
Satie Takahashi
Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14040-901 Ribeirão
Preto, SP, Brazil; Department of Gynecology and Obstetrics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Av.
Bandeirantes, 3900, 14040-900 Ribeirão Preto, SP, Brazil; Department of Biology, Faculty of Philosophy, Sciences and Letters of
Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900, 14040-901 Ribeirão Preto, SP, Brazil.
Received September 3, 2009; accepted October 13, 2009; available online October 20, 2009
Abstract: The present study evaluated the basal DNA damage and the cellular response to this damage induced by in vitro
administration of Etoposide in lymphocytes donated by twenty untreated breast cancer (BC) patients and twenty age-matched healthy
women. Micronucleus (MN) and alkaline Comet Assays were performed in cultured peripheral blood lymphocytes (PBL) according to a
standard protocol for in vitro treatment with various concentrations of Etoposide or a control. For the Comet Assay, three samples of
cells were collected: T0 (immediately preceding treatment of the cultures), T1 (immediately after completion of the treatment) and T2
(four hours after completion of the treatment). MN frequency in the BC group treated with 25 µM Etoposide (19.1 ± 7.35) was
significantly higher than the control (10.9 ± 9.87) group. In the alkaline Comet Assay, both the BC group and the healthy women showed
the ability to repair Etoposide-induced DNA damage within 4 hours of reincubation. (IJCEM909002).
Key words: Micronucleus Test, comet Assay, breast cancer, DNA repair, Etoposide
Full Text PDF
Address all correspondence to:
Department of Genetics
Faculty of Medicine of Ribeirão Preto
University of São Paulo
Av. Bandeirantes, 3900, 14040-901
Ribeirão Preto, SP, Brazil.
E-mail: anaclaudia.teixeira@yahoo.com.br
