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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2(3):193-202;2009
Review Article
Impact of the next generation DNA sequencers
Kikuya Kato
Research Institute, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka,
537-8511, Japan.
Received June 20, 2009; accepted June 26, 2009; available online July, 2009
Abstract: New generation sequencers have been developed with a strong impact on genomics. These sequencers are based on a
principle different from the Sanger method, and can sequence one to several million templates in a single run, albeit read length is
relatively small. The current large-scale efforts are: 1) complete genome sequencing of 1,000 individuals, the primary objective of which
is identification of rare SNP variants, not identified by the international HapMap project; 2) large-scale sequencing of cancer genomes
to construct a complete catalog of genomic changes. These sequencers are also being applied in the identification of new infectious
agents. Steady increase in data production capacity and decrease of cost will definitely make the sequencers a powerful diagnostic
tool, especially for screening of all genetic diseases. On the contrary, statistical problems inherent to large data sets need to be solved
before application to specific problems in medical science. (IJCEM906002).
Key Words: Massive parallel analysis, the 1000 genomes project, the cancer genome atlas
Full Text PDF
Address all correspondence to:
Kikuya Kato, MD
Research Institute
Osaka Medical Center for Cancer and Cardiovascular Diseases
1-3-3 Nakamichi, Higashinari-ku
Osaka, 537-8511
Japan.
E-mail: katou-ki@mc.pref.osaka.jp
Review Article
Impact of the next generation DNA sequencers
Kikuya Kato
Research Institute, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3 Nakamichi, Higashinari-ku, Osaka,
537-8511, Japan.
Received June 20, 2009; accepted June 26, 2009; available online July, 2009
Abstract: New generation sequencers have been developed with a strong impact on genomics. These sequencers are based on a
principle different from the Sanger method, and can sequence one to several million templates in a single run, albeit read length is
relatively small. The current large-scale efforts are: 1) complete genome sequencing of 1,000 individuals, the primary objective of which
is identification of rare SNP variants, not identified by the international HapMap project; 2) large-scale sequencing of cancer genomes
to construct a complete catalog of genomic changes. These sequencers are also being applied in the identification of new infectious
agents. Steady increase in data production capacity and decrease of cost will definitely make the sequencers a powerful diagnostic
tool, especially for screening of all genetic diseases. On the contrary, statistical problems inherent to large data sets need to be solved
before application to specific problems in medical science. (IJCEM906002).
Key Words: Massive parallel analysis, the 1000 genomes project, the cancer genome atlas
Full Text PDF
Address all correspondence to:
Kikuya Kato, MD
Research Institute
Osaka Medical Center for Cancer and Cardiovascular Diseases
1-3-3 Nakamichi, Higashinari-ku
Osaka, 537-8511
Japan.
E-mail: katou-ki@mc.pref.osaka.jp
