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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2(2),131-148;2009
Original Article
Characterization of human septic sera induced gene expression modulation in
human myocytes
Shaimaa Hussein, Paul Michael, Danielle Brabant, Abdelwahab Omri, Ravin Narain, Kalpdrum Passi, Chilakamarti V. Ramana,
Joseph E. Parrillo, Anand Kumar, Amadeo Parissenti, Aseem Kumar
Department of Chemistry and Biochemistry and the Biomolecular Sciences Programme, Department of Mathematics and Computer
Science, Laurentian University, Sudbury, ON, Canada, P3E 2C6. Department of Medicine, Dartmouth-Hitchcock Medical Center,
Lebanon, NH, USA, 03766. Division of Cardiovascular Disease and Critical Care Medicine, Cooper University Hospital, Robert Wood
Johnson Medical School, Camden, New Jersey, USA, 08103. Section of Critical Care Medicine, University of Manitoba, Winnipeg, MB,
Canada, R3A 1R9.
Received February 18, 2009; accepted February, 2009; available online March, 2009
Abstract: To gain a better understanding of the gene expression changes that occurs during sepsis, we have performed a cDNA
microarray study utilizing a tissue culture model that mimics human sepsis. This study utilized an in vitro model of cultured human
fetal cardiac myocytes treated with 10% sera from septic patients or 10% sera from healthy volunteers. A 1700 cDNA expression
microarray was used to compare the transcription profile from human cardiac myocytes treated with septic sera vs normal sera. Septic
sera treatment of myocytes resulted in the down-regulation of 178 genes and the up-regulation of 4 genes. Our data indicate that septic
sera induced cell cycle, metabolic, transcription factor and apoptotic gene expression changes in human myocytes. Identification and
characterization of gene expression changes that occur during sepsis may lead to the development of novel therapeutics and
diagnostics..(IJCEM902003).
Key Words: Septic sera, gene expression modulation, human myocytes
Full Text PDF
Address all correspondence to:
Aseem Kumar PhD
Department of Chemistry and Biochemistry
Laurentian University
935 Ramsey Lake Rd.
Sudbury, ON, Canada
P3E 2C6
Tel: 705-675-1151 ext. 2103
Fax: 705-675-4844
akumar@laurentian.ca
Original Article
Characterization of human septic sera induced gene expression modulation in
human myocytes
Shaimaa Hussein, Paul Michael, Danielle Brabant, Abdelwahab Omri, Ravin Narain, Kalpdrum Passi, Chilakamarti V. Ramana,
Joseph E. Parrillo, Anand Kumar, Amadeo Parissenti, Aseem Kumar
Department of Chemistry and Biochemistry and the Biomolecular Sciences Programme, Department of Mathematics and Computer
Science, Laurentian University, Sudbury, ON, Canada, P3E 2C6. Department of Medicine, Dartmouth-Hitchcock Medical Center,
Lebanon, NH, USA, 03766. Division of Cardiovascular Disease and Critical Care Medicine, Cooper University Hospital, Robert Wood
Johnson Medical School, Camden, New Jersey, USA, 08103. Section of Critical Care Medicine, University of Manitoba, Winnipeg, MB,
Canada, R3A 1R9.
Received February 18, 2009; accepted February, 2009; available online March, 2009
Abstract: To gain a better understanding of the gene expression changes that occurs during sepsis, we have performed a cDNA
microarray study utilizing a tissue culture model that mimics human sepsis. This study utilized an in vitro model of cultured human
fetal cardiac myocytes treated with 10% sera from septic patients or 10% sera from healthy volunteers. A 1700 cDNA expression
microarray was used to compare the transcription profile from human cardiac myocytes treated with septic sera vs normal sera. Septic
sera treatment of myocytes resulted in the down-regulation of 178 genes and the up-regulation of 4 genes. Our data indicate that septic
sera induced cell cycle, metabolic, transcription factor and apoptotic gene expression changes in human myocytes. Identification and
characterization of gene expression changes that occur during sepsis may lead to the development of novel therapeutics and
diagnostics..(IJCEM902003).
Key Words: Septic sera, gene expression modulation, human myocytes
Full Text PDF
Address all correspondence to:
Aseem Kumar PhD
Department of Chemistry and Biochemistry
Laurentian University
935 Ramsey Lake Rd.
Sudbury, ON, Canada
P3E 2C6
Tel: 705-675-1151 ext. 2103
Fax: 705-675-4844
akumar@laurentian.ca
