 | |  | |  | |  | |  | |  | |  | |  |
| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 1(3):283-293;2008
Original Article
Neuroprotective Activities of Enzymatically Hydrolyzed Peptides from Porcine Hide
Gelatin
Shaoyun Wang, Deng-Shun Wang, Rui Wang
College of Bioscience & Biotechnology, Fuzhou University, Fuzhou 350002, China; Shanghai Institute of Materia Medica, Chinese
Academy of Sciences, Shanghai 201203, China, and University of Wisconsin, School of Medicine and Public Health, Madison, WI
53706, USA
Received June 18, 2008; accepted July 30, 2008; available online August 10, 2008
Abstract: Central nervous system disorders, including cerebrovascular disease, neurodegenerative diseases and head trauma are
the most common cause of severe disability in adults and share a number of pathophysiological features. The therapeutic strategy of
neuroprotection has been well accepted as one of the promising approaches in treating such brain disorders, and searching for the
effective neuroprotective agents is still an open-ended task for neurologists and neuro-pharmacologists. In this study, we report for the
first time that the enzymatic hydrolysates from type-B porcine hide gelatin has potent neuroprotective activity against H2O2- or serum
deprivation-induced injuries of cultured SH-SY5Y cells. The peptides used in this study were prepared from type-B porcine hide gelatin
digested with pepsin and papain. The neuroprotective activity of the porcine hide gelatin hydrolysate (PHH) was evaluated using MTT
reduction assay. From the pre-screening of PHH, we found that the whole porcine hide gelatin hydrolysate obtained from papain
digestion (PHH-I) showed significant neuroprotective activities (P<0.05). After further separation of PPH-I through SP-Sephadex C-50
and Sephadex G-25, only the fraction with smaller molecular weight from Sephadex G-25 (PHH-Ic) demonstrated potent
neuroprotective activities (P<0.01). The active fraction showed a molecular mass between 1,000-3,000Da in SDS-polyacrylamide gel
electrophoresis, and was rich in Glycine, Proline and Hydroxyproline in amino acid composition, indicating that peptides with a
spectrum of molecular sizes and certain amino acids are critical for the neuroprotective activities of gelatin peptides. The viability of
cultured cells treated with gelatin peptides was significantly improved in a dose-dependent manner. Further studies are necessary to
establish the neuroprotective activity of hydrolyzed peptides for the neurons in vivo. (IJCEM806003).
Key Words: Gelatin, hydrolysate, antioxidative, neuroprotective, neurotrophic
Full Text PDF
Address all correspondence to: Rui Wang, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese
Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, P.R. China. Email:
wangrui_shanghai@hotmail.com
Original Article
Neuroprotective Activities of Enzymatically Hydrolyzed Peptides from Porcine Hide
Gelatin
Shaoyun Wang, Deng-Shun Wang, Rui Wang
College of Bioscience & Biotechnology, Fuzhou University, Fuzhou 350002, China; Shanghai Institute of Materia Medica, Chinese
Academy of Sciences, Shanghai 201203, China, and University of Wisconsin, School of Medicine and Public Health, Madison, WI
53706, USA
Received June 18, 2008; accepted July 30, 2008; available online August 10, 2008
Abstract: Central nervous system disorders, including cerebrovascular disease, neurodegenerative diseases and head trauma are
the most common cause of severe disability in adults and share a number of pathophysiological features. The therapeutic strategy of
neuroprotection has been well accepted as one of the promising approaches in treating such brain disorders, and searching for the
effective neuroprotective agents is still an open-ended task for neurologists and neuro-pharmacologists. In this study, we report for the
first time that the enzymatic hydrolysates from type-B porcine hide gelatin has potent neuroprotective activity against H2O2- or serum
deprivation-induced injuries of cultured SH-SY5Y cells. The peptides used in this study were prepared from type-B porcine hide gelatin
digested with pepsin and papain. The neuroprotective activity of the porcine hide gelatin hydrolysate (PHH) was evaluated using MTT
reduction assay. From the pre-screening of PHH, we found that the whole porcine hide gelatin hydrolysate obtained from papain
digestion (PHH-I) showed significant neuroprotective activities (P<0.05). After further separation of PPH-I through SP-Sephadex C-50
and Sephadex G-25, only the fraction with smaller molecular weight from Sephadex G-25 (PHH-Ic) demonstrated potent
neuroprotective activities (P<0.01). The active fraction showed a molecular mass between 1,000-3,000Da in SDS-polyacrylamide gel
electrophoresis, and was rich in Glycine, Proline and Hydroxyproline in amino acid composition, indicating that peptides with a
spectrum of molecular sizes and certain amino acids are critical for the neuroprotective activities of gelatin peptides. The viability of
cultured cells treated with gelatin peptides was significantly improved in a dose-dependent manner. Further studies are necessary to
establish the neuroprotective activity of hydrolyzed peptides for the neurons in vivo. (IJCEM806003).
Key Words: Gelatin, hydrolysate, antioxidative, neuroprotective, neurotrophic
Full Text PDF
Address all correspondence to: Rui Wang, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese
Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, P.R. China. Email:
wangrui_shanghai@hotmail.com
