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Int J Clin Exp Med 2013;6(10):942-950
Original Article
Effects of pneumonia and malnutrition on the frequency of micronuclei in peripheral
blood of pediatric patients
Khalid I Elsayh, Douaa M Sayed, Asmaa M Zahran, Khaled Saad, Gamel Badr
Department of Pediatrics, Assiut University, Assiut 71516, Egypt; Department of Clinical Pathology, South Egypt Cancer Institute, Assiut
University, Assiut 71516, Egypt; Princess Al-Johara Al-Ibrahim Center for Cancer Research, College of Medicine, King Saud University,
Riyadh, Saudi Arabia; Laboratory of Immunology & Molecular Biology, Department of Zoology, Faculty of Science, Assiut University,
71516, Assiut, Egypt
Received September 21, 2013; Accepted October 21, 2013; Epub October 25, 2013; Published October 30, 2013
Abstract: The aim of this study was to evaluate the effects of bacterial pneumonia and malnutrition on the frequency of micronuclei
(MN) in peripheral blood of pediatric patients through flow cytometric analysis. The study was an analytical case-control study carried
out on 35 malnourished children with bacterial pneumonia and 20 well-nourished children with bacterial pneumonia, in addition to 20
healthy children as controls. Complete physical examination including; anthropometric measurement, Chest roentgenograms were
done for all cases. Assessment of MN was done by FACSCalibur flow cytometry. The frequency of micronucleated reticulocytes
(MN-RETs) was higher both in the malnourished children with pneumonia and well-nourished children with pneumonia than the
controls. Within the malnourished children with pneumonia, patients with kwashiorkor had more micronucleated mature erythrocytes
(MN-RBCs) and MN-RETs than patients with marasmus. In conclusion: Pneumonia is associated with an increased frequency of MN
and this increment is more pronounced in children with severe malnutrition especially kwashiorkor group. (IJCEM1309017).
Keywords: Pneumonia, malnutrition, micronuclei, flow cytometry, children
Address correspondence to: Dr. Khaled Saad, Department of Pediatrics, Faculty of Medicine, Assiut University, Assiut 71516, Egypt.
Tel: +20-100-608-0182; Fax: +20-88-236-8371; E-mail: ksaad8@yahoo.com
Original Article
Effects of pneumonia and malnutrition on the frequency of micronuclei in peripheral
blood of pediatric patients
Khalid I Elsayh, Douaa M Sayed, Asmaa M Zahran, Khaled Saad, Gamel Badr
Department of Pediatrics, Assiut University, Assiut 71516, Egypt; Department of Clinical Pathology, South Egypt Cancer Institute, Assiut
University, Assiut 71516, Egypt; Princess Al-Johara Al-Ibrahim Center for Cancer Research, College of Medicine, King Saud University,
Riyadh, Saudi Arabia; Laboratory of Immunology & Molecular Biology, Department of Zoology, Faculty of Science, Assiut University,
71516, Assiut, Egypt
Received September 21, 2013; Accepted October 21, 2013; Epub October 25, 2013; Published October 30, 2013
Abstract: The aim of this study was to evaluate the effects of bacterial pneumonia and malnutrition on the frequency of micronuclei
(MN) in peripheral blood of pediatric patients through flow cytometric analysis. The study was an analytical case-control study carried
out on 35 malnourished children with bacterial pneumonia and 20 well-nourished children with bacterial pneumonia, in addition to 20
healthy children as controls. Complete physical examination including; anthropometric measurement, Chest roentgenograms were
done for all cases. Assessment of MN was done by FACSCalibur flow cytometry. The frequency of micronucleated reticulocytes
(MN-RETs) was higher both in the malnourished children with pneumonia and well-nourished children with pneumonia than the
controls. Within the malnourished children with pneumonia, patients with kwashiorkor had more micronucleated mature erythrocytes
(MN-RBCs) and MN-RETs than patients with marasmus. In conclusion: Pneumonia is associated with an increased frequency of MN
and this increment is more pronounced in children with severe malnutrition especially kwashiorkor group. (IJCEM1309017).
Keywords: Pneumonia, malnutrition, micronuclei, flow cytometry, children
Address correspondence to: Dr. Khaled Saad, Department of Pediatrics, Faculty of Medicine, Assiut University, Assiut 71516, Egypt.
Tel: +20-100-608-0182; Fax: +20-88-236-8371; E-mail: ksaad8@yahoo.com
