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Int J Clin Exp Med 2013;6(10):967-972
Original Article
Polymorphisms in the promoters of MMP-2 and TIMP-2 genes in patients with acne
vulgaris
Kursat Oguz Yaykasli, Hakan Turan, Ertugrul Kaya, Omer Faruk Hatipoglu
Department of Medical Genetics, Faculty of Medicine, Duzce University, Duzce, Turkey; Department of Dermatology, Faculty of Medicine,
Duzce University, Duzce, Turkey; Department of Pharmacology, Faculty of Medicine, Duzce University, Duzce, Turkey; Department of
Pathology, Faculty of Medicine, Pittsburg University, Pittsburgh, USA
Received September 17, 2013; Accepted October 2, 2013; Epub October 25, 2013; Published October 30, 2013
Abstract: Acne, a chronic inflammatory skin disease, can be seen at any age but it most often occurs in adolescents and young
people. Several factors, including increased sebum production, abnormal cornification of the pilosebaceous units, proliferation of
Propionibacterium acne, and extracellular matrix (ECM) remodeling, are thought to be associated with the pathogenesis of the acne.
The remodeling of the ECM is regulated by a balance between matrix metalloproteinases (MMPs) and their inhibitors called tissue
inhibitors of metalloproteinases (TIMPs). The current study investigated the potential association between MMP-2 (-1306 C/T) and
TIMP-2 (-418 G/C) polymorphisms and the risk for acne in a Turkish population. The study was conducted with 85 subjects who
presented to the Dermatology Department of Duzce University Hospital. DNA was isolated from 2 ml of peripheral blood taken from
each subject, and their genotypes were analyzed with the polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP) method. The CC, CT, and TT genotypes for MMP-2 (-1306 C/T) polymorphism were similar between the patient and
control group (24 [55.8%], 17 [39.5%], and 2 [4.7%], respectively, vs. 21 [50%], 18 [42.9%], and 3 [7.1%], respectively). However, the
distribution of the GG, GC, and CC genotypes for TIMP-2 (-418 G/C) polymorphism were different between the patient and control group
(30 [69.8%], 9 [14.8%] and 4 [9.3%], respectively, vs. 26 [61.9%], 14 [33.3%], and 2 [4.8%], respectively). The results demonstrated that
the TIMP-2 (-418 CC) genotype was nearly two times more common in the patient group compared to the control group (p=0.686,
OR=1.45). It may be possible that the TIMP-2 (-418 CC) genotype increases the tendency to develop acne vulgaris by disrupting the
balance between MMPs and TIMPs. Further investigations are needed to clarify more precisely the relationship between acne and
MMP-TIMP genes. (IJCEM1309009).
Keywords: Acne vulgaris, MMPs, TIMPs, polymorphism
Address correspondence to: Dr. Kursat Oguz Yaykasli, Department of Medical Genetics, Faculty of Medicine, Duzce University,
Konuralp Campus, 81620, Duzce, Turkey. Tel: +90 380 542 11 00 / 4160; Fax: +90 380 542 13 02; E-mail: kursatyay@yahoo.com
Original Article
Polymorphisms in the promoters of MMP-2 and TIMP-2 genes in patients with acne
vulgaris
Kursat Oguz Yaykasli, Hakan Turan, Ertugrul Kaya, Omer Faruk Hatipoglu
Department of Medical Genetics, Faculty of Medicine, Duzce University, Duzce, Turkey; Department of Dermatology, Faculty of Medicine,
Duzce University, Duzce, Turkey; Department of Pharmacology, Faculty of Medicine, Duzce University, Duzce, Turkey; Department of
Pathology, Faculty of Medicine, Pittsburg University, Pittsburgh, USA
Received September 17, 2013; Accepted October 2, 2013; Epub October 25, 2013; Published October 30, 2013
Abstract: Acne, a chronic inflammatory skin disease, can be seen at any age but it most often occurs in adolescents and young
people. Several factors, including increased sebum production, abnormal cornification of the pilosebaceous units, proliferation of
Propionibacterium acne, and extracellular matrix (ECM) remodeling, are thought to be associated with the pathogenesis of the acne.
The remodeling of the ECM is regulated by a balance between matrix metalloproteinases (MMPs) and their inhibitors called tissue
inhibitors of metalloproteinases (TIMPs). The current study investigated the potential association between MMP-2 (-1306 C/T) and
TIMP-2 (-418 G/C) polymorphisms and the risk for acne in a Turkish population. The study was conducted with 85 subjects who
presented to the Dermatology Department of Duzce University Hospital. DNA was isolated from 2 ml of peripheral blood taken from
each subject, and their genotypes were analyzed with the polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP) method. The CC, CT, and TT genotypes for MMP-2 (-1306 C/T) polymorphism were similar between the patient and
control group (24 [55.8%], 17 [39.5%], and 2 [4.7%], respectively, vs. 21 [50%], 18 [42.9%], and 3 [7.1%], respectively). However, the
distribution of the GG, GC, and CC genotypes for TIMP-2 (-418 G/C) polymorphism were different between the patient and control group
(30 [69.8%], 9 [14.8%] and 4 [9.3%], respectively, vs. 26 [61.9%], 14 [33.3%], and 2 [4.8%], respectively). The results demonstrated that
the TIMP-2 (-418 CC) genotype was nearly two times more common in the patient group compared to the control group (p=0.686,
OR=1.45). It may be possible that the TIMP-2 (-418 CC) genotype increases the tendency to develop acne vulgaris by disrupting the
balance between MMPs and TIMPs. Further investigations are needed to clarify more precisely the relationship between acne and
MMP-TIMP genes. (IJCEM1309009).
Keywords: Acne vulgaris, MMPs, TIMPs, polymorphism
Address correspondence to: Dr. Kursat Oguz Yaykasli, Department of Medical Genetics, Faculty of Medicine, Duzce University,
Konuralp Campus, 81620, Duzce, Turkey. Tel: +90 380 542 11 00 / 4160; Fax: +90 380 542 13 02; E-mail: kursatyay@yahoo.com
