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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2013;6(8):614-620
Original Article
Experimental observation of in vitro pigs’ hearts with bipolar radiofrequency
ablation
Min Li, Yingmin Chen, Zhangyuan Luo, Wenzan Zhang, Ben He
Department of Cardiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Jie Sheng (Shanghai)
Biological Technology Co., Ltd, Shanghai, China
Received June 13, 2013; Accepted August 15, 2013; Epub September 1, 2013; Published September 15, 2013
Abstract: Bipolar ablation is an innovative direction of catheter ablation technology. The aim of this study was to investigate the
damaged area of bipolar radiofrequency ablation in vitro pigs’ hearts under different conditions and to evaluate the effectiveness and
safety. The bipolar radiofrequency ablation was performed in vitro pigs’ hearts with a 4 mm bipolar electrode under different conditions
at various combinations of the parameters (Distance: from 12 mm to 17 mm; Power: 30 w or 40 w; Time: from 20 s to 90 s;
Temperature: 45°C or 60°C; Saline Perfusion: 0 ml/h, 1000 ml/h or 1500 ml/h). We measured the length, width, depth, connection rate
and blasting rate of the connective ablation lesion and then evaluated the effectiveness and safety of connective ablation lesion.
Numerical analysis showed that there was a positive correlation between the time, temperature and the length, depth, connection rate,
blasting rate. There was a negative correlation between the power, perfusion and the depth, while other indexes were positively
correlated with the power, perfusion. Distance and length were positively correlated, while other indexes were negatively correlated with
the distance. Regression analysis showed that the infusion was not significantly correlated with the depth, connection rate, blasting
rate. The temperature was not significantly correlated with the length, connection rate. Power and depth’s correlation was not
significant. When the parameter combination was 12 mm, 20 s, 30 w, 60°C, 1500 ml/h, the effectiveness and safety was optimum, with
the mean lesion length being 19.89 ± 3.02 mm, the depth of an average being 3.50 ± 0.63 mm, the connection rate and the blasting
rate being 100% and 13.9% respectively. The bipolar ablation in vitro pigs’ hearts could form the most stable continuous damage.
(IJCEM1306012).
Keywords: Bipolar ablation, isolated pigs’ hearts, cold saline perfusion, connective ablation
Address correspondence to: Dr. Yingmin Chen, Department of Cardiology, Renji Hospital, School of medicine, Shanghai Jiao Tong
University, No. 5 Building, 145 Shandongzhong Road, Shanghai 200001, China. Tel: 0086-136-81980901; Fax: 0086-021-34611387; E-
mail: chenyingmin@medmail.com.cn
Original Article
Experimental observation of in vitro pigs’ hearts with bipolar radiofrequency
ablation
Min Li, Yingmin Chen, Zhangyuan Luo, Wenzan Zhang, Ben He
Department of Cardiology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Jie Sheng (Shanghai)
Biological Technology Co., Ltd, Shanghai, China
Received June 13, 2013; Accepted August 15, 2013; Epub September 1, 2013; Published September 15, 2013
Abstract: Bipolar ablation is an innovative direction of catheter ablation technology. The aim of this study was to investigate the
damaged area of bipolar radiofrequency ablation in vitro pigs’ hearts under different conditions and to evaluate the effectiveness and
safety. The bipolar radiofrequency ablation was performed in vitro pigs’ hearts with a 4 mm bipolar electrode under different conditions
at various combinations of the parameters (Distance: from 12 mm to 17 mm; Power: 30 w or 40 w; Time: from 20 s to 90 s;
Temperature: 45°C or 60°C; Saline Perfusion: 0 ml/h, 1000 ml/h or 1500 ml/h). We measured the length, width, depth, connection rate
and blasting rate of the connective ablation lesion and then evaluated the effectiveness and safety of connective ablation lesion.
Numerical analysis showed that there was a positive correlation between the time, temperature and the length, depth, connection rate,
blasting rate. There was a negative correlation between the power, perfusion and the depth, while other indexes were positively
correlated with the power, perfusion. Distance and length were positively correlated, while other indexes were negatively correlated with
the distance. Regression analysis showed that the infusion was not significantly correlated with the depth, connection rate, blasting
rate. The temperature was not significantly correlated with the length, connection rate. Power and depth’s correlation was not
significant. When the parameter combination was 12 mm, 20 s, 30 w, 60°C, 1500 ml/h, the effectiveness and safety was optimum, with
the mean lesion length being 19.89 ± 3.02 mm, the depth of an average being 3.50 ± 0.63 mm, the connection rate and the blasting
rate being 100% and 13.9% respectively. The bipolar ablation in vitro pigs’ hearts could form the most stable continuous damage.
(IJCEM1306012).
Keywords: Bipolar ablation, isolated pigs’ hearts, cold saline perfusion, connective ablation
Address correspondence to: Dr. Yingmin Chen, Department of Cardiology, Renji Hospital, School of medicine, Shanghai Jiao Tong
University, No. 5 Building, 145 Shandongzhong Road, Shanghai 200001, China. Tel: 0086-136-81980901; Fax: 0086-021-34611387; E-
mail: chenyingmin@medmail.com.cn
