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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2013;6(5):404-412
Original Article
Expressional time phase of leukocyte molecules induced by allogenic cardiac
antigen and cyclosporin A in rats’ in vitro model
Xiu-Fang Xu, Yi Xin, Ying Zhang, Yi-Min Huang, Yun Gu, Na Li, Wen-Bin Li, Yu-Jie Zhou, Zhao-Guang Zhang
Department of Molecular Biology, Capital Medical University Affiliated with Beijing Anzhen Hospital, Beijing Institute of Heart, Lung &
Blood Vessel Diseases, Beijing 100029, China; Department of Cardiac Surgery, Capital Medical University Affiliated with Beijing
Anzhen Hospital, Beijing 100029, China. Wen-Bin Li contributed equally to this paper.
Received April 11, 2013; Accepted April 26, 2013; Epub May 22, 2013; Published June 1, 2013
Abstract: The immunosuppressive agent cyclosporin A has been proven to reduce the rejection rate and prolong the survival time of
transplanted hearts. But some reports showed that cyclosporine A did not completely suppress the rejection. We performed in vitro
studies to model a time course to observe the effect of cyclosporin A. Methods: The experiment was divided into a control group (group
I), an antigen group (group II), a cyclosporin A group (group III) and an antigen + cyclosporin A group (group IV). After transplantation, at
2 h, 6 h, 12 h, 24 h, 48 h and 72 h, leukocyte molecules were monitored. Results: The expression of IL-2R peaked at 12 h in group II
and at 6 h in group III. There was a gradual decline in the expression of the P59 gene in group I, positive expression at 2 h and between
12 h and 24 h in group II, in group IV, there was a decrease at 48 h. The expression of the CD4 gene was lowest at 2 h in group I and at
6 h in group II. CD4 expression then quickly increased to a maximum at 48 h in group III, at 2 h in group IV. There was a minimal
expression was reached at 12 h in group I and IV and at 6 h in group III in the expression of the CD8 gene. Conclusions: Alloantigen
induced lymphocytes to release IL-2R and P59 and stimulated the induction of the CD4 gene’ transcription for 6 h. Cyclosporin A
stimulated the release of IL-2R for 2 h. These results provide an in vitro basis for describing the time phases of rejection inhibited by
cyclosporin A. (IJCEM1304010).
Keywords: Transplantation rejection model in vitro, early expression’ time phase, cyclosporin A
Address correspondence to: Zhao-Guang Zhang, Department of Cardiac Surgery, Institute of Heart, Lung and Blood Vessels, Anzhen
Hospital, Capital Medical University, Beijing 100029, China. Tel: 86-10-64456652; Fax: 86-010-64444521; E-mail: ssnfder@163.com.
Co-Correspondence: Yi-Min Huang, E-mail: yiminh0504@sina.com
Original Article
Expressional time phase of leukocyte molecules induced by allogenic cardiac
antigen and cyclosporin A in rats’ in vitro model
Xiu-Fang Xu, Yi Xin, Ying Zhang, Yi-Min Huang, Yun Gu, Na Li, Wen-Bin Li, Yu-Jie Zhou, Zhao-Guang Zhang
Department of Molecular Biology, Capital Medical University Affiliated with Beijing Anzhen Hospital, Beijing Institute of Heart, Lung &
Blood Vessel Diseases, Beijing 100029, China; Department of Cardiac Surgery, Capital Medical University Affiliated with Beijing
Anzhen Hospital, Beijing 100029, China. Wen-Bin Li contributed equally to this paper.
Received April 11, 2013; Accepted April 26, 2013; Epub May 22, 2013; Published June 1, 2013
Abstract: The immunosuppressive agent cyclosporin A has been proven to reduce the rejection rate and prolong the survival time of
transplanted hearts. But some reports showed that cyclosporine A did not completely suppress the rejection. We performed in vitro
studies to model a time course to observe the effect of cyclosporin A. Methods: The experiment was divided into a control group (group
I), an antigen group (group II), a cyclosporin A group (group III) and an antigen + cyclosporin A group (group IV). After transplantation, at
2 h, 6 h, 12 h, 24 h, 48 h and 72 h, leukocyte molecules were monitored. Results: The expression of IL-2R peaked at 12 h in group II
and at 6 h in group III. There was a gradual decline in the expression of the P59 gene in group I, positive expression at 2 h and between
12 h and 24 h in group II, in group IV, there was a decrease at 48 h. The expression of the CD4 gene was lowest at 2 h in group I and at
6 h in group II. CD4 expression then quickly increased to a maximum at 48 h in group III, at 2 h in group IV. There was a minimal
expression was reached at 12 h in group I and IV and at 6 h in group III in the expression of the CD8 gene. Conclusions: Alloantigen
induced lymphocytes to release IL-2R and P59 and stimulated the induction of the CD4 gene’ transcription for 6 h. Cyclosporin A
stimulated the release of IL-2R for 2 h. These results provide an in vitro basis for describing the time phases of rejection inhibited by
cyclosporin A. (IJCEM1304010).
Keywords: Transplantation rejection model in vitro, early expression’ time phase, cyclosporin A
Address correspondence to: Zhao-Guang Zhang, Department of Cardiac Surgery, Institute of Heart, Lung and Blood Vessels, Anzhen
Hospital, Capital Medical University, Beijing 100029, China. Tel: 86-10-64456652; Fax: 86-010-64444521; E-mail: ssnfder@163.com.
Co-Correspondence: Yi-Min Huang, E-mail: yiminh0504@sina.com
