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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2013;6(3):166-173
Original Article
Electrophysiology properties of voltage-gated potassium channels in rat peritoneal
macrophages
Bao-Ming Wu, Xiao-Hua Wang, Bin Zhao, Er-Bao Bian, Huang Yan, Huang Cheng, Xiong-Wen Lv, Zhi-Gang Xiong, Jun Li
School of Pharmacy, Anhui Medical University, Heifei, 230032, Anhui Province, China; Deputy Chief, Tong Guan Shan, Tongling city
,244000, Anhui Province, China; Neuroscience Institute, Morehouse School of Medicine, Atlanta, GA 30310, USA
Received January 20, 2013; Accepted February 23, 2013; Epub March 21, 2013; Published March 31, 2013
Abstract: Ion channels are important for the functions of excitable and non-excitable cells. Using the whole-cell patch clamp technique,
we analyzed the electrophysiological and pharmacological properties of voltage-gated potassium channels in primary rat peritoneal
macrophages. With intracellular solution contained K+ as the main charge carrier, all cells showed outward currents in response to
membrane depolarization. The currents can be inhibited by TEA (10 mM), a non-selective blocker for voltage-gated K+ channels, and
attenuated when intracellular K+ was substituted with Cs+. Changing holding potential from -80 to -30 mV or -10 mV also inhibited the
outward currents. In contrast, increasing the concentration of ATP in the intracellular solution decreased the amplitude of the outward
currents. Thus, rat peritoneal macrophages express several types of functional voltage-gated K+ channels. (IJCEM1301008).
Keywords: Patch-clamp, peritoneal macrophage, potassium channel, TEA, ATP
Address correspondence to: Dr. Jun Li, School of Pharmacy, Anhui Medical University, Hefei 230032, Anhui Province, China China.
E-mail: lijun@ahmu.edu.cn; Dr. Zhigang Xiong, Neuroscience Institute, Morehouse School of Medicine, Atlanta, GA 30310, USA. E-mail:
zxiong@msm.edu
Original Article
Electrophysiology properties of voltage-gated potassium channels in rat peritoneal
macrophages
Bao-Ming Wu, Xiao-Hua Wang, Bin Zhao, Er-Bao Bian, Huang Yan, Huang Cheng, Xiong-Wen Lv, Zhi-Gang Xiong, Jun Li
School of Pharmacy, Anhui Medical University, Heifei, 230032, Anhui Province, China; Deputy Chief, Tong Guan Shan, Tongling city
,244000, Anhui Province, China; Neuroscience Institute, Morehouse School of Medicine, Atlanta, GA 30310, USA
Received January 20, 2013; Accepted February 23, 2013; Epub March 21, 2013; Published March 31, 2013
Abstract: Ion channels are important for the functions of excitable and non-excitable cells. Using the whole-cell patch clamp technique,
we analyzed the electrophysiological and pharmacological properties of voltage-gated potassium channels in primary rat peritoneal
macrophages. With intracellular solution contained K+ as the main charge carrier, all cells showed outward currents in response to
membrane depolarization. The currents can be inhibited by TEA (10 mM), a non-selective blocker for voltage-gated K+ channels, and
attenuated when intracellular K+ was substituted with Cs+. Changing holding potential from -80 to -30 mV or -10 mV also inhibited the
outward currents. In contrast, increasing the concentration of ATP in the intracellular solution decreased the amplitude of the outward
currents. Thus, rat peritoneal macrophages express several types of functional voltage-gated K+ channels. (IJCEM1301008).
Keywords: Patch-clamp, peritoneal macrophage, potassium channel, TEA, ATP
Address correspondence to: Dr. Jun Li, School of Pharmacy, Anhui Medical University, Hefei 230032, Anhui Province, China China.
E-mail: lijun@ahmu.edu.cn; Dr. Zhigang Xiong, Neuroscience Institute, Morehouse School of Medicine, Atlanta, GA 30310, USA. E-mail:
zxiong@msm.edu
