 | |  | |  | |  | |  | |  | |  | |  |
| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2013;6(3):192-196
Original Article
Leptin mediated ObRb receptor increases expression of adhesion in-tercellular
molecules and cyclooxygenase 2 on murine aorta tissue inducing endothelial
dysfunction
Leticia Manuel-Apolinar, Ricardo López-Romero, Arturo Zarate, Leticia Damasio, Miriam Ruiz, Carmen Castillo-Hernández, Gustavo
Guevara, Elvia Mera-Jiménez
Endocrine Research Unit, Centro Medico Nacional, Instituto Mexicano del Seguro Social (IMSS), Cuauhtemoc 330, Doctores, Mexico
City 06270, Mexico; Research and Graduate Studies Section, Instituto Politecnico Nacional (IPN), Cuauhtemoc 330, Doctores, Mexico
City 06270, Mexico; Oncology Research Unit, Centro Medico Nacional, Instituto Mexicano del Seguro Social (IMSS), Cuauhtemoc 330,
Doctores, Mexico City 06270, Mexico
Received January 8, 2013; Accepted March 2, 2013; Epub March 21, 2013; Published March 31, 2013
Abstract: There are several reports showing that hyperleptinemia positively correlates with atherogenic process including promotion of
platelet aggregation, thrombosis, and production of inflammatory cytokines. Thereby endothelial dysfunction takes place and underlies
metabolic and vascular alterations that contribute to the development of both cardiovascular disease and type 2 diabetes. It has been
also proposed that endothelial dysfunction may antecede the development of insulin resistance and diabetes. Endothelial cells
participate in vasoregulation by the modulation of nitric oxide production and prostaglandin; in addition these cells are major vector in
angiogenesis and recruitment of leukocytes and adhesion molecules. Nevertheless mechanisms underlying vascular dysfunction
remain to be fully elucidated. This experiment in vitro revealed that the addition of leptin to cultivated endothelial cells elicited a
significant molecular expression of both COX 2 and ICAM-1: in addition, the response showed a positive relationship with leptin
concentration and the time of incubation. Thus, it may be suggested that leptin acts directly on the endothelium by activating its specific
receptor which in turn initiates the molecular response related with the production of factors involved in the inflammatory response.
Alterations on prostaglandins and recruiting molecules of adhesion are relevant stages of the endothelial damage. (IJCEM1301004).
Keywords: Endothelium, leptin, receptor Ob, ICAM-1, COX-2
Address correspondence to: Leticia Manuel-Apolinar, Endocrine Research Unit, Centro Medico Nacional, Instituto Mexicano del
Seguro Social (IMSS), Cuauhtemoc 330, Doctores, Mexico City 06270, Mexico. E-mail: letymanu@yahoo.com.mx
Original Article
Leptin mediated ObRb receptor increases expression of adhesion in-tercellular
molecules and cyclooxygenase 2 on murine aorta tissue inducing endothelial
dysfunction
Leticia Manuel-Apolinar, Ricardo López-Romero, Arturo Zarate, Leticia Damasio, Miriam Ruiz, Carmen Castillo-Hernández, Gustavo
Guevara, Elvia Mera-Jiménez
Endocrine Research Unit, Centro Medico Nacional, Instituto Mexicano del Seguro Social (IMSS), Cuauhtemoc 330, Doctores, Mexico
City 06270, Mexico; Research and Graduate Studies Section, Instituto Politecnico Nacional (IPN), Cuauhtemoc 330, Doctores, Mexico
City 06270, Mexico; Oncology Research Unit, Centro Medico Nacional, Instituto Mexicano del Seguro Social (IMSS), Cuauhtemoc 330,
Doctores, Mexico City 06270, Mexico
Received January 8, 2013; Accepted March 2, 2013; Epub March 21, 2013; Published March 31, 2013
Abstract: There are several reports showing that hyperleptinemia positively correlates with atherogenic process including promotion of
platelet aggregation, thrombosis, and production of inflammatory cytokines. Thereby endothelial dysfunction takes place and underlies
metabolic and vascular alterations that contribute to the development of both cardiovascular disease and type 2 diabetes. It has been
also proposed that endothelial dysfunction may antecede the development of insulin resistance and diabetes. Endothelial cells
participate in vasoregulation by the modulation of nitric oxide production and prostaglandin; in addition these cells are major vector in
angiogenesis and recruitment of leukocytes and adhesion molecules. Nevertheless mechanisms underlying vascular dysfunction
remain to be fully elucidated. This experiment in vitro revealed that the addition of leptin to cultivated endothelial cells elicited a
significant molecular expression of both COX 2 and ICAM-1: in addition, the response showed a positive relationship with leptin
concentration and the time of incubation. Thus, it may be suggested that leptin acts directly on the endothelium by activating its specific
receptor which in turn initiates the molecular response related with the production of factors involved in the inflammatory response.
Alterations on prostaglandins and recruiting molecules of adhesion are relevant stages of the endothelial damage. (IJCEM1301004).
Keywords: Endothelium, leptin, receptor Ob, ICAM-1, COX-2
Address correspondence to: Leticia Manuel-Apolinar, Endocrine Research Unit, Centro Medico Nacional, Instituto Mexicano del
Seguro Social (IMSS), Cuauhtemoc 330, Doctores, Mexico City 06270, Mexico. E-mail: letymanu@yahoo.com.mx
