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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2013;6(3):185-191
Original Article
Effect of gastric acidification on the 14C-UBT HELIPROBE® accuracy during
Pantoprazole treatment in Helicobacter pylori positive patients
Fariborz Mansour-Ghanaei, Farahnaz Joukar, Mohammad Reza Sheykhian, Fatemeh Soati, Ali Mohammad Rafatzand
Division of Gastroenterology & Hepatology, Gastrointestinal & Liver Diseases Research Center (GLDRC), Guilan University of Medical
Sciences, Rasht, Iran; Gastrointestinal & Liver Diseases Research Center (GLDRC), Guilan University of Medical Sciences, Rasht,
Iran; Division of Gastroenterology & Hepatology, Gastrointestinal & Liver Diseases Research Center (GLDRC), Guilan University of
Medical Sciences, Rasht, Iran; Gastrointestinal & Liver Diseases Research Center (GLDRC), Guilan University of Medical Sciences,
Rasht, Iran; Chemistry, Sharif Institute of technology, Tehran, Iran
Received November 17, 2012; Accepted February 3, 2013; Epub March 21, 2013; Published March 31, 2013
Abstract: The aim of this study was to evaluate the influence of using Citric Acid on false negative rates induced by PPIs during 14C–
UBT in dyspeptic patients with H. pylori infection. In a crossover randomized controlled clinical trial, one hundred dyspeptic patients (46
females and 54 males) with determined H. pylori infection who referred to gastrointestinal Outpatient Clinic of Razi Hospital-Rasht,
Iran. All the patients underwent a 14C-UBT HELIPROBE® baseline test and the positive ones entered the second phase. They were
divided randomly to two groups and started PPIs treatment by Pantoprazole (20 mg/daily Pantozol® Nycomed Company) and
underwent two other UBTs in days 12-13 and 14-15 with and without 4 grams Citric Acid. In group I who underwent UBT with Citric Acid
in days 12-13 (UBT2) and UBT without Citric Acid in days 14-15 (UBT3), there was no significant difference between the UBT results
with and without Citric Acid (P≥0.05). In group II who underwent UBT without Citric Acid in days 12-13 and UBT with Citric Acid in days
14-15, the false negative rates were not significantly different between UBT with and without Citric Acid (P≥0.05). In both groups the
results of UBT without Citric Acid after Pantoprazole consumption didn’t change significantly compared to the baseline UBT. Also the
difference between the false negative rates of baseline UBT and UBT without citric acid was not significant (P>0.05). These results
suggest that acidification of gastric environment during 14C-UBT cannot prevent false negative results and do not increase the
accuracy of the test in patients taking PPIs. (IJCEM1211007).
Keywords: Helicobacter pylori, urea breath test, citric acid
Address correspondence to: Dr. Fariborz Mansour-Ghanaei, Gastrointestinal and Liver Diseases Research Center (GLDRC) Razi
hospital, Sardar-Jangle Ave, PO Box: 41448 -95655, Rasht, Iran. Phone: +98-131-535116; Fax: +98-131-5534951; E-mail:
ghanaei@gums.ac.ir or ghanaie@yahoo.com
Original Article
Effect of gastric acidification on the 14C-UBT HELIPROBE® accuracy during
Pantoprazole treatment in Helicobacter pylori positive patients
Fariborz Mansour-Ghanaei, Farahnaz Joukar, Mohammad Reza Sheykhian, Fatemeh Soati, Ali Mohammad Rafatzand
Division of Gastroenterology & Hepatology, Gastrointestinal & Liver Diseases Research Center (GLDRC), Guilan University of Medical
Sciences, Rasht, Iran; Gastrointestinal & Liver Diseases Research Center (GLDRC), Guilan University of Medical Sciences, Rasht,
Iran; Division of Gastroenterology & Hepatology, Gastrointestinal & Liver Diseases Research Center (GLDRC), Guilan University of
Medical Sciences, Rasht, Iran; Gastrointestinal & Liver Diseases Research Center (GLDRC), Guilan University of Medical Sciences,
Rasht, Iran; Chemistry, Sharif Institute of technology, Tehran, Iran
Received November 17, 2012; Accepted February 3, 2013; Epub March 21, 2013; Published March 31, 2013
Abstract: The aim of this study was to evaluate the influence of using Citric Acid on false negative rates induced by PPIs during 14C–
UBT in dyspeptic patients with H. pylori infection. In a crossover randomized controlled clinical trial, one hundred dyspeptic patients (46
females and 54 males) with determined H. pylori infection who referred to gastrointestinal Outpatient Clinic of Razi Hospital-Rasht,
Iran. All the patients underwent a 14C-UBT HELIPROBE® baseline test and the positive ones entered the second phase. They were
divided randomly to two groups and started PPIs treatment by Pantoprazole (20 mg/daily Pantozol® Nycomed Company) and
underwent two other UBTs in days 12-13 and 14-15 with and without 4 grams Citric Acid. In group I who underwent UBT with Citric Acid
in days 12-13 (UBT2) and UBT without Citric Acid in days 14-15 (UBT3), there was no significant difference between the UBT results
with and without Citric Acid (P≥0.05). In group II who underwent UBT without Citric Acid in days 12-13 and UBT with Citric Acid in days
14-15, the false negative rates were not significantly different between UBT with and without Citric Acid (P≥0.05). In both groups the
results of UBT without Citric Acid after Pantoprazole consumption didn’t change significantly compared to the baseline UBT. Also the
difference between the false negative rates of baseline UBT and UBT without citric acid was not significant (P>0.05). These results
suggest that acidification of gastric environment during 14C-UBT cannot prevent false negative results and do not increase the
accuracy of the test in patients taking PPIs. (IJCEM1211007).
Keywords: Helicobacter pylori, urea breath test, citric acid
Address correspondence to: Dr. Fariborz Mansour-Ghanaei, Gastrointestinal and Liver Diseases Research Center (GLDRC) Razi
hospital, Sardar-Jangle Ave, PO Box: 41448 -95655, Rasht, Iran. Phone: +98-131-535116; Fax: +98-131-5534951; E-mail:
ghanaei@gums.ac.ir or ghanaie@yahoo.com
