IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
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Int J Clin Exp Med 2013;6(1):57-66
Original Article
Assessment of possible association between rs3787016 and prostate cancer risk in
Serbian population
Zorana Z Nikolić, Goran N Brajušković, Dušanka Lj Savić Pavićević, Aleksandar S Kojić, Vinka D Vukotić, Saša M Tomović, Snežana J
Cerović, Vladimir Filipović, Đuro Mišljenović, Stanka P Romac
University of Belgrade, Faculty of Biology, Belgrade, Serbia; Department of Urology, Clinical Centre “dr Dragiša Mišović”, Belgrade,
Serbia; Clinical of Surgery, Clinical Centre “Zvezdara”, Belgrade, Serbia; Institute of Pathology and Forensic Medicine, Military Medical
Academy, Belgrade, Serbia; University of Belgrade, Faculty of Mathematics, Belgrade, Serbia
Received October 3, 2012; accepted November 2, 2012; Epub November 18, 2012; Published January 1, 2012
Abstract: Recent study, which included meta-analysis of two genome-wide association studies (GWAS), followed by a replication,
identified the association between single nucleotide polymorphism (SNP) rs3787016 at 19p13 and prostate cancer (PCa) risk.
Considering possible genetic differences between populations, we conducted the study in order to evaluate the association of this
polymorphism with prostate cancer risk in Serbian population. 261 samples of peripheral blood were obtained from the patients with
PCa and 257 samples from patients with benign prostatic hyperplasia (BPH). 106 volunteers who gave samples of bucal swabs
comprised the control group. For individuals diagnosed with PCa clinicopathological characteristics including serum prostate-specific
antigen (PSA) level at diagnosis, Gleason score (GS) and clinical stage were determined. Genotypization of rs3787016 was performed
by using Taqman® SNP Genotyping Assay. The differences in alelle and genotype frequencies between analyzed groups of subjects
were performed by using PLINK, SPSS 17.0 for Windows and SNPStats statistical software. No significant association of rs3787016
with PCa risk was determined comparing allele and genotype frequencies among group of patients diagnosed with PCa and the
control group, as well as among groups of patients with PCa and BPH. Also, no evidence of association of rs3787016 with PCa risk
was shown using tests for association under dominant and recessive genetic models. SNP rs3787016 showed no significant
association with standard prognostic parameters regarding PCa progression, nor with the risk of disease progression assessed
according to two different risk classification systems. (IJCEM1210001).
Keywords: Prostate cancer, association study, single nucleotide polymorphism (SNP)
Address all correspondence to:
Dr. Goran Brajušković
Studentski trg 3, P. Box 52
11000 Belgrade, Serbia.
Tel: + 381 11 2639 100; Fax: + 381 11 26 39 100
E-mail: brajuskovic@bio.bg.ac.rs