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Int J Clin Exp Med 2013;6(1):16-25
Original Article
JAK kinases are required for the bacterial RNA and poly I:C induced tyrosine
phosphorylation of PKR
Farag Bleiblo, Paul Michael, Danielle Brabant, Chilakamarti V Ramana, TC Tai, Mazen Saleh, Joseph E Parrillo, Anand Kumar, Aseem
Kumar
Department of Chemistry and Biochemistry and the Biomolecular Sciences Programme, Laurentian University, Sudbury, ON, Canada,
P3E 2C6; Department of Biology, University of Benghazi, Benghazi, Libya; Department of Medicine, Yale University, New Haven, CT,
USA, 06516; Division of Cardiovascular Disease and Critical Care Medicine, Cooper University Hospital, Robert Wood Johnson
Medical School, Camden, New Jersey, USA, 08103; Section of Critical Care Medicine, University of Manitoba, Winnipeg, MB, Canada,
R3A 1R9
Received September 10, 2012; accepted November 1, 2012; Epub November 18, 2012; Published January 1, 2013
Abstract: Discriminating the molecular patterns associated with RNA is central to innate immunity. The protein kinase PKR is a
cytosolic sensor involved in the recognition of viral dsRNA and triggering interferon-induced signaling. Here, we identified bacterial RNA
as a novel distinct pattern recognized by PKR. We show that the tyrosine phosphorylation of PKR induced by either bacterial RNA or poly
I:C is impaired in mutant cells lacking TYK2, JAK1, or JAK2 kinases. PKR was found to be a direct substrate for the activated JAKs. Our
results indicated that the double-stranded structures of bacterial RNA are required to fully activate PKR. These results suggest that
bacterial RNA signaling is analogous in some respects to that of viral RNA and interferons and may have implications in bacterial
immunity. (IJCM1209003).
Keywords: PKR, JAKS, tyrosine phosphorylation, bacterial RNA, innate immunity
Address all correspondence to:
Dr. Aseem Kumar
Department of Chemistry and Biochemistry
Biomolecular Sciences Programme
Laurentian University, 935 Ramsey Lake Rd.
Sudbury, ON, Canada, P3E 2C6.
Tel: 705-675-1151 ext.2103; Fax: 705-675-4844
E-mail: akumar@laurentian.ca
Original Article
JAK kinases are required for the bacterial RNA and poly I:C induced tyrosine
phosphorylation of PKR
Farag Bleiblo, Paul Michael, Danielle Brabant, Chilakamarti V Ramana, TC Tai, Mazen Saleh, Joseph E Parrillo, Anand Kumar, Aseem
Kumar
Department of Chemistry and Biochemistry and the Biomolecular Sciences Programme, Laurentian University, Sudbury, ON, Canada,
P3E 2C6; Department of Biology, University of Benghazi, Benghazi, Libya; Department of Medicine, Yale University, New Haven, CT,
USA, 06516; Division of Cardiovascular Disease and Critical Care Medicine, Cooper University Hospital, Robert Wood Johnson
Medical School, Camden, New Jersey, USA, 08103; Section of Critical Care Medicine, University of Manitoba, Winnipeg, MB, Canada,
R3A 1R9
Received September 10, 2012; accepted November 1, 2012; Epub November 18, 2012; Published January 1, 2013
Abstract: Discriminating the molecular patterns associated with RNA is central to innate immunity. The protein kinase PKR is a
cytosolic sensor involved in the recognition of viral dsRNA and triggering interferon-induced signaling. Here, we identified bacterial RNA
as a novel distinct pattern recognized by PKR. We show that the tyrosine phosphorylation of PKR induced by either bacterial RNA or poly
I:C is impaired in mutant cells lacking TYK2, JAK1, or JAK2 kinases. PKR was found to be a direct substrate for the activated JAKs. Our
results indicated that the double-stranded structures of bacterial RNA are required to fully activate PKR. These results suggest that
bacterial RNA signaling is analogous in some respects to that of viral RNA and interferons and may have implications in bacterial
immunity. (IJCM1209003).
Keywords: PKR, JAKS, tyrosine phosphorylation, bacterial RNA, innate immunity
Address all correspondence to:
Dr. Aseem Kumar
Department of Chemistry and Biochemistry
Biomolecular Sciences Programme
Laurentian University, 935 Ramsey Lake Rd.
Sudbury, ON, Canada, P3E 2C6.
Tel: 705-675-1151 ext.2103; Fax: 705-675-4844
E-mail: akumar@laurentian.ca
