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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2012;5(2):195-200
Original Article
Comparison of adenosine deaminase levels in serum and synovial fluid between
patients with rheumatoid arthritis and osteoarthritis
Zahra Zakeri, Abassali Niazi, Mansoor Shakiba, Behzad Narouie, Zohre Bari, Soodabeh Zendeboodi, Mohammad Ghasemi-Rad
Department of Internal Medicine, Ali- ebne -Abitaleb Hospital, Zahedan University of medical sciences, Zahedan, Iran; Clinical
Research Development Center, Ali-ebne-Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran; Genius and
Talented Student Organization, Student Research Committee (SRC), Urmia University of Medical Sciences, Urmia, Iran
Received January 24; accepted March 27; Epub April 6, 2012; Published April 30, 2012
Abstract: Objectives: Adenosine deaminase (ADA) is an enzyme being involved in purine metabolism and plays a significant role in the
immune system. The aim of this study was to investigate the use of adenosine deaminase levels in differentiating between rheumatoid
arthritis and osteoarthritis. Material and methods: Thirty patients with rheumatoid arthritis and 30 osteoarthritis patients enrolled the
study. They were matched in sex and age. Using the ROC curve, we determined the optimal serum and synovial cutoff for rheumatoid
effusion. Results: The results showed a statistically significant difference between ADA levels in joint effusion and serum of patients
with rheumatoid arthritis and osteoarthritis (p<0.001). Synovial fluid cutoff value for diagnosing rheumatoid arthritis was 20 with a
sensitivity of 90% and specificity of 80% and the serum cutoff value was 15 with a sensitivity of 93% and specificity of 53.3%. Area under
ROC curve for synovial ADA, ESR and CRP co-linearity was 99%. Conclusion: We concluded that synovial total ADA assay can be a
sensitive and specific test, being suitable for rapid diagnosis of rheumatoid effusions. (IJCEM1201006).
Keywords: Adenosine deaminase (ADA), enzyme, purine metabolism, immune system, rheumatoid arthritis, serum, synovial fluid,
osteoarthritis, human
Address all correspondence to:
Dr. Mohammad Ghasemi-Rad
Genius and Talented Student Organization
Student Research Committee (SRC)
Urmia University of Medical Sciences
Urmia, Iran.
Tel: 00989143485284; Fax: 00982353561
E-mail: medman11@gmail.com
Original Article
Comparison of adenosine deaminase levels in serum and synovial fluid between
patients with rheumatoid arthritis and osteoarthritis
Zahra Zakeri, Abassali Niazi, Mansoor Shakiba, Behzad Narouie, Zohre Bari, Soodabeh Zendeboodi, Mohammad Ghasemi-Rad
Department of Internal Medicine, Ali- ebne -Abitaleb Hospital, Zahedan University of medical sciences, Zahedan, Iran; Clinical
Research Development Center, Ali-ebne-Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran; Genius and
Talented Student Organization, Student Research Committee (SRC), Urmia University of Medical Sciences, Urmia, Iran
Received January 24; accepted March 27; Epub April 6, 2012; Published April 30, 2012
Abstract: Objectives: Adenosine deaminase (ADA) is an enzyme being involved in purine metabolism and plays a significant role in the
immune system. The aim of this study was to investigate the use of adenosine deaminase levels in differentiating between rheumatoid
arthritis and osteoarthritis. Material and methods: Thirty patients with rheumatoid arthritis and 30 osteoarthritis patients enrolled the
study. They were matched in sex and age. Using the ROC curve, we determined the optimal serum and synovial cutoff for rheumatoid
effusion. Results: The results showed a statistically significant difference between ADA levels in joint effusion and serum of patients
with rheumatoid arthritis and osteoarthritis (p<0.001). Synovial fluid cutoff value for diagnosing rheumatoid arthritis was 20 with a
sensitivity of 90% and specificity of 80% and the serum cutoff value was 15 with a sensitivity of 93% and specificity of 53.3%. Area under
ROC curve for synovial ADA, ESR and CRP co-linearity was 99%. Conclusion: We concluded that synovial total ADA assay can be a
sensitive and specific test, being suitable for rapid diagnosis of rheumatoid effusions. (IJCEM1201006).
Keywords: Adenosine deaminase (ADA), enzyme, purine metabolism, immune system, rheumatoid arthritis, serum, synovial fluid,
osteoarthritis, human
Address all correspondence to:
Dr. Mohammad Ghasemi-Rad
Genius and Talented Student Organization
Student Research Committee (SRC)
Urmia University of Medical Sciences
Urmia, Iran.
Tel: 00989143485284; Fax: 00982353561
E-mail: medman11@gmail.com
