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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2011;4(1):67-73
Original Article
Cell type-specific release of matrix-metallo-proteinase-9 by bacterial
chemoattractant in human blood phagocytic leukocytes
Astrid M. Doerner, Ling-Yu Chen, Richard D. Ye, Jiang Yong, Shuang Huang, Zhixing K. Pan
Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, OH 43614, USA; Department of
Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA; Department of Pathophysiology and Key Laboratory of
Proteomics of Guangdong Province, Southern Medical University, Guangzhou, P.R. China; 4Department of Molecular and Experimental
Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Received January 11, 2011; accepted January 28, 2011; Epub February 6, 2011; published February 28, 2011
Abstract: Stimulation of phagocytic leukocytes with bacterial chemoattractant resulted in the release of matrix metalloproteinases
(MMPs). Little is known about the mechanisms of bacterial chemoattractant regulation of MMP in phagocytic leukocytes. We report here
that the mechanisms of the bacterial chemotactic peptide fMLP-induced MMP-9 release in monocytes appeared to be different from
fMLP-stimulated MMP-9 release in neutrophils. In freshly prepared peripheral blood monocytes, fMLP induces MMP-9 release, starting
at 8 h after stimulation. These functions of fMLP is accompanied by an increase in TNFα expression, and mediated through the
phosphorylation of ERK1/2 in monocytes. However, neutrophil preparations that responded to fMLP with MMP-9 release did not require
activation of ERK1/2 and TNFα expression. These results suggest a different role of fMLP in MMP-9 expression in neutrophils and
monocytes, and the signal molecules involved in mediating this effect in human blood monocytes stimulated by bacterial
chemoattractant. (IJCEM1101002).
Keywords: Bacterial chemoattractant, inflammation, phagocytic leukocytes, matrix-metallo-proteinase
Full Text PDF
Address all correspondence to:
Zhixing K. Pan, MD, PhD
Department of Medical Microbiology and Immunology
Medical University of Ohio, 3035 Arlington Avenue
Toledo, OH 43614, USA.
Tel: (419) 383-5466, Fax: (419) 383-3002
E-mail: kevin.pan@utoledo.edu
Original Article
Cell type-specific release of matrix-metallo-proteinase-9 by bacterial
chemoattractant in human blood phagocytic leukocytes
Astrid M. Doerner, Ling-Yu Chen, Richard D. Ye, Jiang Yong, Shuang Huang, Zhixing K. Pan
Department of Medical Microbiology and Immunology, Medical University of Ohio, Toledo, OH 43614, USA; Department of
Pharmacology, University of Illinois at Chicago, Chicago, IL 60612, USA; Department of Pathophysiology and Key Laboratory of
Proteomics of Guangdong Province, Southern Medical University, Guangzhou, P.R. China; 4Department of Molecular and Experimental
Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
Received January 11, 2011; accepted January 28, 2011; Epub February 6, 2011; published February 28, 2011
Abstract: Stimulation of phagocytic leukocytes with bacterial chemoattractant resulted in the release of matrix metalloproteinases
(MMPs). Little is known about the mechanisms of bacterial chemoattractant regulation of MMP in phagocytic leukocytes. We report here
that the mechanisms of the bacterial chemotactic peptide fMLP-induced MMP-9 release in monocytes appeared to be different from
fMLP-stimulated MMP-9 release in neutrophils. In freshly prepared peripheral blood monocytes, fMLP induces MMP-9 release, starting
at 8 h after stimulation. These functions of fMLP is accompanied by an increase in TNFα expression, and mediated through the
phosphorylation of ERK1/2 in monocytes. However, neutrophil preparations that responded to fMLP with MMP-9 release did not require
activation of ERK1/2 and TNFα expression. These results suggest a different role of fMLP in MMP-9 expression in neutrophils and
monocytes, and the signal molecules involved in mediating this effect in human blood monocytes stimulated by bacterial
chemoattractant. (IJCEM1101002).
Keywords: Bacterial chemoattractant, inflammation, phagocytic leukocytes, matrix-metallo-proteinase
Full Text PDF
Address all correspondence to:
Zhixing K. Pan, MD, PhD
Department of Medical Microbiology and Immunology
Medical University of Ohio, 3035 Arlington Avenue
Toledo, OH 43614, USA.
Tel: (419) 383-5466, Fax: (419) 383-3002
E-mail: kevin.pan@utoledo.edu
