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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2011;4(1):81-90
Original Article
Mesenchymal stem cells display different gene expression profiles compared to
hyaline and elastic chondrocytes
Li-Jie Zhai, Ke-Qing Zhao, Zhi-Qiang Wang, Ya Feng, Shuang-Chun Xing
Department of Otolaryngology–Head and Neck Surgery, First affiliated hospital of Dalian Medical University, Dalian 116011, China;
Department of Otolaryngology–Head and Neck Surgery, Eye and ENT Hospital, School of Shanghai Medicine, Fudan University,
Shanghai 200031, China.
Received January 9, 2011; accepted February 9, 2011; Epub February 10, 2011; published February 28, 2011
Abstract: Stimulation of phagocytic leukocytes with bacterial chemoattractant resulted in the release of matrix metalloproteinases
(MMPs). Little is known about the mechanisms of bacterial chemoattractant regulation of MMP in phagocytic leukocytes. We report here
that the mechanisms of the bacterial chemotactic peptide fMLP-induced MMP-9 release in monocytes appeared to be different from
fMLP-stimulated MMP-9 release in neutrophils. In freshly prepared peripheral blood monocytes, fMLP induces MMP-9 release, starting
at 8 h after stimulation. These functions of fMLP is accompanied by an increase in TNFα expression, and mediated through the
phosphorylation of ERK1/2 in monocytes. However, neutrophil preparations that responded to fMLP with MMP-9 release did not require
activation of ERK1/2 and TNFα expression. These results suggest a different role of fMLP in MMP-9 expression in neutrophils and
monocytes, and the signal molecules involved in mediating this effect in human blood monocytes stimulated by bacterial
chemoattractant. (IJCEM1101001).
Keywords: Bacterial chemoattractant, inflammation, phagocytic leukocytes, matrix-metallo-proteinase
Full Text PDF
Address all correspondence to:
Li-Jie Zhai, MD
Department of Otolaryngology–Head and Neck Surgery
First affiliated hospital of Dalian Medical University
222 Zhongshan RD, Dalian, China 116011.
Tel: 86-411-82182498; Fax: 86-411-83673291
E-mail: lijiezhai@yahoo.com.cn
Original Article
Mesenchymal stem cells display different gene expression profiles compared to
hyaline and elastic chondrocytes
Li-Jie Zhai, Ke-Qing Zhao, Zhi-Qiang Wang, Ya Feng, Shuang-Chun Xing
Department of Otolaryngology–Head and Neck Surgery, First affiliated hospital of Dalian Medical University, Dalian 116011, China;
Department of Otolaryngology–Head and Neck Surgery, Eye and ENT Hospital, School of Shanghai Medicine, Fudan University,
Shanghai 200031, China.
Received January 9, 2011; accepted February 9, 2011; Epub February 10, 2011; published February 28, 2011
Abstract: Stimulation of phagocytic leukocytes with bacterial chemoattractant resulted in the release of matrix metalloproteinases
(MMPs). Little is known about the mechanisms of bacterial chemoattractant regulation of MMP in phagocytic leukocytes. We report here
that the mechanisms of the bacterial chemotactic peptide fMLP-induced MMP-9 release in monocytes appeared to be different from
fMLP-stimulated MMP-9 release in neutrophils. In freshly prepared peripheral blood monocytes, fMLP induces MMP-9 release, starting
at 8 h after stimulation. These functions of fMLP is accompanied by an increase in TNFα expression, and mediated through the
phosphorylation of ERK1/2 in monocytes. However, neutrophil preparations that responded to fMLP with MMP-9 release did not require
activation of ERK1/2 and TNFα expression. These results suggest a different role of fMLP in MMP-9 expression in neutrophils and
monocytes, and the signal molecules involved in mediating this effect in human blood monocytes stimulated by bacterial
chemoattractant. (IJCEM1101001).
Keywords: Bacterial chemoattractant, inflammation, phagocytic leukocytes, matrix-metallo-proteinase
Full Text PDF
Address all correspondence to:
Li-Jie Zhai, MD
Department of Otolaryngology–Head and Neck Surgery
First affiliated hospital of Dalian Medical University
222 Zhongshan RD, Dalian, China 116011.
Tel: 86-411-82182498; Fax: 86-411-83673291
E-mail: lijiezhai@yahoo.com.cn
