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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2010;3(2):144-151
Original Article
Oxidative stress induced in rat brain by a combination of 3-nitropropionic acid and
global ischemia
Dusan Dobrota, Tatiana N. Fedorova, Maria S. Stepanova, Eva Babusikova, Dagmar Statelova, Zuzana Tatarkova, Sergey S. Stvolinsky,
Alexander A. Boldyrev
Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic; Laboratory of
Clinical Neurochemistry, Research Center of Neurology RAMS, Moscow, Russia; International Center for Biotechnology, Lomonosov
Moscow State University, Moscow, Russia; Clinic of Stomatology and Maxillofacial Surgery, Jessenius Faculty of Medicine, Comenius
University, Martin, Slovak Republic.
Received February 25, 2010, accepted May 11, 2010, available online May 15, 2010
Abstract: In our investigation, we describe the complex model of brain oxidative stress consisted of combination of experimental brain
ischemia and energy metabolism violation induced by irreversible inhibitor of mitochondrial succinate dehydrogenase,
3-nitropropionate (3-NPA). 3-NPA causes selective degeneration of striatum neurons, which is extremely sensitive to energy deficit.
This complex model allows revealing not only biochemical but also neurological symptoms in experimental animals that permits
proper estimation of protective effect of different drugs on animal status. Combination of global ischemia induced by 3-vessel occlusion
of major arteries supplys rat brain and subsequent 5-day reperfusion with intraperitoneal injection of 3-nitropropionic acid induces
vigorous oxidative stress in brain tissues accompanied by evident neurological symptoms in Wistar rats. Such a combination of
damaging factors may be considered as a new complex experimental model of brain oxidative stress permitting the evaluation of
neuroprotective effect of potential therapeutic agents. Using this model, protective effect of neuropeptide carnosine was demonstrated
which is in agreement with previous data. (IJCEM1002006).
Key words: Brain global ischemia, 3-nitropropionic acid, oxidative stress, carnosine
Full Text PDF
Address all correspondence to:
Stepanova Maria, PhD
Research Center of Neurology RAMS
Moscow, Russia.
Tel: +7(495) 4902409, Fax: +7(495) 4902409
E-mail: stepanova.mar@gmail.com
Original Article
Oxidative stress induced in rat brain by a combination of 3-nitropropionic acid and
global ischemia
Dusan Dobrota, Tatiana N. Fedorova, Maria S. Stepanova, Eva Babusikova, Dagmar Statelova, Zuzana Tatarkova, Sergey S. Stvolinsky,
Alexander A. Boldyrev
Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius University, Martin, Slovak Republic; Laboratory of
Clinical Neurochemistry, Research Center of Neurology RAMS, Moscow, Russia; International Center for Biotechnology, Lomonosov
Moscow State University, Moscow, Russia; Clinic of Stomatology and Maxillofacial Surgery, Jessenius Faculty of Medicine, Comenius
University, Martin, Slovak Republic.
Received February 25, 2010, accepted May 11, 2010, available online May 15, 2010
Abstract: In our investigation, we describe the complex model of brain oxidative stress consisted of combination of experimental brain
ischemia and energy metabolism violation induced by irreversible inhibitor of mitochondrial succinate dehydrogenase,
3-nitropropionate (3-NPA). 3-NPA causes selective degeneration of striatum neurons, which is extremely sensitive to energy deficit.
This complex model allows revealing not only biochemical but also neurological symptoms in experimental animals that permits
proper estimation of protective effect of different drugs on animal status. Combination of global ischemia induced by 3-vessel occlusion
of major arteries supplys rat brain and subsequent 5-day reperfusion with intraperitoneal injection of 3-nitropropionic acid induces
vigorous oxidative stress in brain tissues accompanied by evident neurological symptoms in Wistar rats. Such a combination of
damaging factors may be considered as a new complex experimental model of brain oxidative stress permitting the evaluation of
neuroprotective effect of potential therapeutic agents. Using this model, protective effect of neuropeptide carnosine was demonstrated
which is in agreement with previous data. (IJCEM1002006).
Key words: Brain global ischemia, 3-nitropropionic acid, oxidative stress, carnosine
Full Text PDF
Address all correspondence to:
Stepanova Maria, PhD
Research Center of Neurology RAMS
Moscow, Russia.
Tel: +7(495) 4902409, Fax: +7(495) 4902409
E-mail: stepanova.mar@gmail.com
