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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2(4):354-362;2009.
Original Article
Continuous assessment of concentrations of cytokines in experimental injuries of
the extremity
Simon Farnebo, Karlander, Lars-Eril, Steinwall Ingrid, Florence Sjögren, Sjöberg Folke
Departments of Plastic Surgery, Hand Surgery and Burns, and Intensive Care, Clinical and Experimental Medicine, Division of
Dermatology, Faculty of Health Sciences, Linköping University Hospital, S-581 85, Linköping, Sweden
Received September 10, 2009; accepted November 23, 2009; available online November 26, 2009
Abstract: Background. Inflammation plays an important part in the healing process. Little is known about the extent local inflammatory
trauma response interacts with the central circulation and inflammation produced by central organs. The aim of the present study was
to examine whether high cut-off microdialysis catheters offer potential to in real time assess interstitial cytokines variations in
conjunction to markers of metabolism distal to a blunt vascular contusion. Methods. In a standardised contusion trauma model,
microdialysis catheters (high MW (100kDa)) were inserted in the gracilis muscle distal to the trauma for the local assessment of IL-6,
IL-8, TNF-α, total protein and the metabolic mediators (glycerol, puruvate and lactate). The contra lateral uninjured leg served
as control of the centrally mediated inflammation propagated to the extremities. Results. The trauma led to a significant and
quantitatively large (8-10 fold) increase in inflammatory cytokines (IL6 and 8) as measured both in the injured and control legs. There
was only a minor, and not significant increase in concentrations of cytokines in the injured leg compared to the control leg.. There were
no signs of ischemia in either leg. Conclusion. The new finding in this study is that both central, and local, inflammatory responses as
well as metabolic mediators may be assessed continuously in skeletal muscle tissue distal to a major injury in an animal model. The
findings suggest that the large trauma elicits a generalised inflammatory response to trauma rather than propagating a local
one distal to the trauma. (IJCEM909005).
Key words: Blunt trauma, microcirculation, inflammation, microdialysis, rat
Full Text PDF
Address all correspondence to:
Folke Sjöberg, MD,
PhD, Departments of Hand, Plastic Surgery and
Burns, Intensive care, Division of Clinical and Experimental
Medicine, Medical Faculty, Linköping
University Hospital, Linköping, Sweden.
Tel: +46 70-5571820, Fax: + 46 13 22 2836
Email: folsj@ibk.liu.se
Original Article
Continuous assessment of concentrations of cytokines in experimental injuries of
the extremity
Simon Farnebo, Karlander, Lars-Eril, Steinwall Ingrid, Florence Sjögren, Sjöberg Folke
Departments of Plastic Surgery, Hand Surgery and Burns, and Intensive Care, Clinical and Experimental Medicine, Division of
Dermatology, Faculty of Health Sciences, Linköping University Hospital, S-581 85, Linköping, Sweden
Received September 10, 2009; accepted November 23, 2009; available online November 26, 2009
Abstract: Background. Inflammation plays an important part in the healing process. Little is known about the extent local inflammatory
trauma response interacts with the central circulation and inflammation produced by central organs. The aim of the present study was
to examine whether high cut-off microdialysis catheters offer potential to in real time assess interstitial cytokines variations in
conjunction to markers of metabolism distal to a blunt vascular contusion. Methods. In a standardised contusion trauma model,
microdialysis catheters (high MW (100kDa)) were inserted in the gracilis muscle distal to the trauma for the local assessment of IL-6,
IL-8, TNF-α, total protein and the metabolic mediators (glycerol, puruvate and lactate). The contra lateral uninjured leg served
as control of the centrally mediated inflammation propagated to the extremities. Results. The trauma led to a significant and
quantitatively large (8-10 fold) increase in inflammatory cytokines (IL6 and 8) as measured both in the injured and control legs. There
was only a minor, and not significant increase in concentrations of cytokines in the injured leg compared to the control leg.. There were
no signs of ischemia in either leg. Conclusion. The new finding in this study is that both central, and local, inflammatory responses as
well as metabolic mediators may be assessed continuously in skeletal muscle tissue distal to a major injury in an animal model. The
findings suggest that the large trauma elicits a generalised inflammatory response to trauma rather than propagating a local
one distal to the trauma. (IJCEM909005).
Key words: Blunt trauma, microcirculation, inflammation, microdialysis, rat
Full Text PDF
Address all correspondence to:
Folke Sjöberg, MD,
PhD, Departments of Hand, Plastic Surgery and
Burns, Intensive care, Division of Clinical and Experimental
Medicine, Medical Faculty, Linköping
University Hospital, Linköping, Sweden.
Tel: +46 70-5571820, Fax: + 46 13 22 2836
Email: folsj@ibk.liu.se
