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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 2(1):36-40;2009
Original Article
Milk fat globule-EGF factor 8 gene expression in rat splanchnic tissues during
postnatal development
Xiao Wang, Heng-Fu Bu, Isabelle G. De Plaen, Xiao-Di Tan
Center for Digestive Diseases and Immunobiology, Children’s Memorial Research Center, Feinberg School of Medicine, Northwestern
University, Chicago, IL 60614-3394; Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, IL
60614.
Received December 17, 2008; accepted January 30, 2009; available online January 31, 2009
Abstract: Milk fat globule-EGF factor (MFG-E8) is protein that binds to αvβ3/5 integrin and phosphatidylserine. It plays a role in apoptotic
cell removal, tissue remodeling, intestinal epithelial wound-healing process. In the present study, we examined the expression of MFG-
E8 mRNA in the small intestine, liver and lungs during postnatal development. Sprague-Dawley rats were sacrificed at different ages
(E20, P0, P5, P10 and P21). Total RNA was extracted from various tissues including the small intestine, liver and lungs. The MFG-E8
gene expression was quantified by real-time PCR. We found that the MFG-E8 gene was expressed at a low level on E20 in the liver.
Within 24 hours after birth, its expression was markedly increased. It was then stably expressed at high level during the postnatal
period. In contrast, in the small intestine, MFG-E8 gene significantly decreased more than 60% (p<0.001) with 24 hours after birth
compared to E20, then it gradually regained E20 values by P10. The gene was persistently expressed until P21. In the lungs, MFG-E8
is constitutively expressed prenatally at E20. Its expression did not change during the postnatal period. In summary, our study indicated
that MFG-E8 is extensively expressed in the small intestine, liver, and lungs. As opposed to other organs, the expression of intestinal
MFG-E8 is decreased during the first week of life. It is possible that this could contribute to the predisposition of the neonatal intestine
to inflammation. (IJCEM812002).
Key Words: MFG-E8, postnatal gene expression, intestines, liver, lung
Full Text PDF
Address all correspondence to:
Xiao-Di Tan, M.D.
Center for Digestive Diseases and Immunobiology
Children's Memorial Research Center
Children’s Memorial Hospital
2300 Children's Plaza, Box 217, Chicago, IL 60614-3363
Tel: (773) 755-6380
Fax: (773) 755-6581
e-mail: xtan@northwestern.edu
Original Article
Milk fat globule-EGF factor 8 gene expression in rat splanchnic tissues during
postnatal development
Xiao Wang, Heng-Fu Bu, Isabelle G. De Plaen, Xiao-Di Tan
Center for Digestive Diseases and Immunobiology, Children’s Memorial Research Center, Feinberg School of Medicine, Northwestern
University, Chicago, IL 60614-3394; Department of Pediatrics, Feinberg School of Medicine, Northwestern University, Chicago, IL
60614.
Received December 17, 2008; accepted January 30, 2009; available online January 31, 2009
Abstract: Milk fat globule-EGF factor (MFG-E8) is protein that binds to αvβ3/5 integrin and phosphatidylserine. It plays a role in apoptotic
cell removal, tissue remodeling, intestinal epithelial wound-healing process. In the present study, we examined the expression of MFG-
E8 mRNA in the small intestine, liver and lungs during postnatal development. Sprague-Dawley rats were sacrificed at different ages
(E20, P0, P5, P10 and P21). Total RNA was extracted from various tissues including the small intestine, liver and lungs. The MFG-E8
gene expression was quantified by real-time PCR. We found that the MFG-E8 gene was expressed at a low level on E20 in the liver.
Within 24 hours after birth, its expression was markedly increased. It was then stably expressed at high level during the postnatal
period. In contrast, in the small intestine, MFG-E8 gene significantly decreased more than 60% (p<0.001) with 24 hours after birth
compared to E20, then it gradually regained E20 values by P10. The gene was persistently expressed until P21. In the lungs, MFG-E8
is constitutively expressed prenatally at E20. Its expression did not change during the postnatal period. In summary, our study indicated
that MFG-E8 is extensively expressed in the small intestine, liver, and lungs. As opposed to other organs, the expression of intestinal
MFG-E8 is decreased during the first week of life. It is possible that this could contribute to the predisposition of the neonatal intestine
to inflammation. (IJCEM812002).
Key Words: MFG-E8, postnatal gene expression, intestines, liver, lung
Full Text PDF
Address all correspondence to:
Xiao-Di Tan, M.D.
Center for Digestive Diseases and Immunobiology
Children's Memorial Research Center
Children’s Memorial Hospital
2300 Children's Plaza, Box 217, Chicago, IL 60614-3363
Tel: (773) 755-6380
Fax: (773) 755-6581
e-mail: xtan@northwestern.edu
