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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 1(4):332-337;2008
Original Article
The Effect of Whole-Body Radiation on Blood Levels of Gastrointestinal Peptides in
the Rat
Sakdhisapol Katanyutanon, Rongqian Wu, and Ping Wang
Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, North Shore University Hospital & Division of Surgical
Research, Department of Surgery, North Shore University Hospital and Long Island Jewish Medical Center & Laboratory of Surgical
Research, The Feinstein Institute for Medical Research, Manhasset, NY 11030, USA
Received October 5, 2008; accepted, October 12, 2008; available online October 15, 2008
Abstract: Radiation-induced injury may occur in various incidents as well as the terrorist radiation exposure scenario. The digestive
tract is among the most radiosensitive organs in the body and its function, which is partly regulated by gastrointestinal (GI) peptides,
can be affected by radiation exposure. However, very little is known about the effect of whole-body radiation on blood GI peptides. The
aim of this study therefore was to determine the effect of whole-body radiation on circulating levels of GI peptides in the rat. To study
this, rats were exposed to 5-Gy whole-body gamma radiation. They were then euthanized at 1, 2, 4, or 8 days after irradiation. Plasma
levels of cholecystokinin (CCK), secretin, gastrin, and ghrelin were determined using specific enzyme immunoassays. Serum levels of
alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin, and lactate were also measured. Our results showed that
whole-body irradiation significantly decreased plasma CCK levels by 57% and 54% at 1 and 2 days after irradiation (P<0.05),
respectively. At 4 and 8 days after irradiation, plasma CCK levels returned to normal. Similarly, plasma levels of secretin decreased by
48% at 2 days after irradiation (P<0.05), and returned to normal at 8 days after irradiation. In contrast, there was no significant change in
plasma levels of gastrin and ghrelin after irradiation. No significant differences were observed in ALT, ALP, total bilirubin, or lactate. In
conclusion, whole-body radiation exposure alters blood GI peptides especially the ones that were produced in the small intestine, such
as CCK and secretin. The diverse response of the GI peptides to irradiation could be due to a difference in radiosensitivity in different
regions of the GI tract. (IJCEM810002).
Key Words: Radiation injury, gastrin, cholecystokinin, secretin, ghrelin
Full Text PDF
Address all correspondence to: Ping Wang, MD, Laboratory of Surgical Research, The Feinstein Institute for Medical Research, 350
Community Drive, Manhasset, NY 11030, Fax: (516) 562-1022, E-mail: pwang@nshs.edu
Original Article
The Effect of Whole-Body Radiation on Blood Levels of Gastrointestinal Peptides in
the Rat
Sakdhisapol Katanyutanon, Rongqian Wu, and Ping Wang
Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, North Shore University Hospital & Division of Surgical
Research, Department of Surgery, North Shore University Hospital and Long Island Jewish Medical Center & Laboratory of Surgical
Research, The Feinstein Institute for Medical Research, Manhasset, NY 11030, USA
Received October 5, 2008; accepted, October 12, 2008; available online October 15, 2008
Abstract: Radiation-induced injury may occur in various incidents as well as the terrorist radiation exposure scenario. The digestive
tract is among the most radiosensitive organs in the body and its function, which is partly regulated by gastrointestinal (GI) peptides,
can be affected by radiation exposure. However, very little is known about the effect of whole-body radiation on blood GI peptides. The
aim of this study therefore was to determine the effect of whole-body radiation on circulating levels of GI peptides in the rat. To study
this, rats were exposed to 5-Gy whole-body gamma radiation. They were then euthanized at 1, 2, 4, or 8 days after irradiation. Plasma
levels of cholecystokinin (CCK), secretin, gastrin, and ghrelin were determined using specific enzyme immunoassays. Serum levels of
alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin, and lactate were also measured. Our results showed that
whole-body irradiation significantly decreased plasma CCK levels by 57% and 54% at 1 and 2 days after irradiation (P<0.05),
respectively. At 4 and 8 days after irradiation, plasma CCK levels returned to normal. Similarly, plasma levels of secretin decreased by
48% at 2 days after irradiation (P<0.05), and returned to normal at 8 days after irradiation. In contrast, there was no significant change in
plasma levels of gastrin and ghrelin after irradiation. No significant differences were observed in ALT, ALP, total bilirubin, or lactate. In
conclusion, whole-body radiation exposure alters blood GI peptides especially the ones that were produced in the small intestine, such
as CCK and secretin. The diverse response of the GI peptides to irradiation could be due to a difference in radiosensitivity in different
regions of the GI tract. (IJCEM810002).
Key Words: Radiation injury, gastrin, cholecystokinin, secretin, ghrelin
Full Text PDF
Address all correspondence to: Ping Wang, MD, Laboratory of Surgical Research, The Feinstein Institute for Medical Research, 350
Community Drive, Manhasset, NY 11030, Fax: (516) 562-1022, E-mail: pwang@nshs.edu
