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| IJCEM Copyright © 2008-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711 |
Int J Clin Exp Med 1(2),145-153;2008
Original Article
Electrophysiological Properties of Mouse Cortical Neuron Progenitors Differentiated
In Vitro and In Vivo
Weizhen Wang, Kunlin Jin, Xiao-Ou Mao, Natasha Close, David A Greenberg, and Zhi-Gang Xiong
Robert S. Dow Neurobiology Laboratories, Legacy Research, Portland, Oregon 97232, USA; 2 Buck Institute for Age Research, Novato,
California 94945, USA
Received January 4, 2008; accepted with revision January 17, 2008; available online March 6, 2008
Abstract: Central neurons are highly vulnerable to injury and have limited ability to regenerate. Therefore, transplantation of exogenous
neuronal progenitor cells has been considered a potential therapy for the restoration of lost neurons and associated brain function. In a
previous study, we found that when injected into rat brain following focal ischemia, cortical neuronal progenitor cells cultured from
mouse brain can migrate into ischemic areas and differentiate into cells with morphological and biochemical features of neurons.
However, no direct electrophysiological evidence was provided to indicate that these cells become functional neurons in vivo. In this
study, we measured the electrophysiological properties of neuronal progenitor cells from embryonic mouse cerebral cortex, both in cell
culture and in rat brain slices following intracerebral injection. We demonstrate that some of these cells differentiate to express
electrophysiological properties expected of mature neurons, including tetrodotoxin-sensitive Na+ channels and N-methyl-D-aspartate
receptor channels. These results support the feasibility of cell-replacement therapy for stroke using exogenous neuronal
progenitors.(IJCEM801001).
Key Words: Progenitor cell; differentiation; neuron; patch-clamp; ion channel; ischemia
Full Text PDF
Address all correspondence to: Dr. Zhi-Gang Xiong, Robert S. Dow Neurobiology Laboratories, Legacy Research , 1225 NE 2nd
Avenue, Portland OR 97232, Tel: (503) 413-2086, Fax: (503) 413-5465, E-mail: zxiong@Downeurobiology.org
Original Article
Electrophysiological Properties of Mouse Cortical Neuron Progenitors Differentiated
In Vitro and In Vivo
Weizhen Wang, Kunlin Jin, Xiao-Ou Mao, Natasha Close, David A Greenberg, and Zhi-Gang Xiong
Robert S. Dow Neurobiology Laboratories, Legacy Research, Portland, Oregon 97232, USA; 2 Buck Institute for Age Research, Novato,
California 94945, USA
Received January 4, 2008; accepted with revision January 17, 2008; available online March 6, 2008
Abstract: Central neurons are highly vulnerable to injury and have limited ability to regenerate. Therefore, transplantation of exogenous
neuronal progenitor cells has been considered a potential therapy for the restoration of lost neurons and associated brain function. In a
previous study, we found that when injected into rat brain following focal ischemia, cortical neuronal progenitor cells cultured from
mouse brain can migrate into ischemic areas and differentiate into cells with morphological and biochemical features of neurons.
However, no direct electrophysiological evidence was provided to indicate that these cells become functional neurons in vivo. In this
study, we measured the electrophysiological properties of neuronal progenitor cells from embryonic mouse cerebral cortex, both in cell
culture and in rat brain slices following intracerebral injection. We demonstrate that some of these cells differentiate to express
electrophysiological properties expected of mature neurons, including tetrodotoxin-sensitive Na+ channels and N-methyl-D-aspartate
receptor channels. These results support the feasibility of cell-replacement therapy for stroke using exogenous neuronal
progenitors.(IJCEM801001).
Key Words: Progenitor cell; differentiation; neuron; patch-clamp; ion channel; ischemia
Full Text PDF
Address all correspondence to: Dr. Zhi-Gang Xiong, Robert S. Dow Neurobiology Laboratories, Legacy Research , 1225 NE 2nd
Avenue, Portland OR 97232, Tel: (503) 413-2086, Fax: (503) 413-5465, E-mail: zxiong@Downeurobiology.org
